Analogous Enzymes: Independent Inventions in Enzyme Evolution
نویسندگان
چکیده
منابع مشابه
Analogous enzymes: independent inventions in enzyme evolution.
It is known that the same reaction may be catalyzed by structurally unrelated enzymes. We performed a systematic search for such analogous (as opposed to homologous) enzymes by evaluating sequence conservation among enzymes with the same enzyme classification (EC) number using sensitive, iterative sequence database search methods. Enzymes without detectable sequence similarity to each other wer...
متن کاملFunctional genomics and enzyme evolution. Homologous and analogous enzymes encoded in microbial genomes.
Computational analysis of complete genomes, followed by experimental testing of emerging hypotheses--the area of research often referred to as 'functional genomics'--aims at deciphering the wealth of information contained in genome sequences and at using it to improve our understanding of the mechanisms of cell function. This review centers on the recent progress in the genome analysis with spe...
متن کاملDe novo design and evolution of artificial disulfide isomerase enzymes analogous to the bacterial DsbC.
The Escherichia coli disulfide isomerase, DsbC is a V-shaped homodimer with each monomer comprising a dimerization region that forms part of a putative peptide-binding pocket and a thioredoxin catalytic domain. Disulfide isomerases from prokaryotes and eukaryotes exhibit little sequence homology but display very similar structural organization with two thioredoxin domains facing each other on t...
متن کاملDiscovery of Analogous Enzymes in Thiamin Biosynthesis by Anticorrelation
1 Instituto de Biotecnoloǵıa, Universidad Nacional Aut. de México, AP510-3, Cuernavaca 62250, Mexico 2 European Molecular Biology Laboratory, Meyerhofstr. 1, 69117 Heidelberg, Germany 3 Max Delbrück Center for Molecular Medicine, 13092 Berlin-Buch, Germany 4 University of Heidelberg, Dept. of Parasitology, INF 324, 69120 Heidelberg, Germany 5 Nijmegen Centre for Molecular Life Sciences, p/a CMB...
متن کاملEvolution of highly active enzymes by homology-independent recombination.
The theta-class GST enzymes hGSTT1-1 (human GSTTheta-1-1) and rGSTT2-2 (rat GSTTheta-2-2) share 54.3% amino acid identity and exhibit different substrate specificities. Homology-independent techniques [incremental truncation for the creation of hybrid enzymes (ITCHY) and SCRATCHY] and low-homology techniques (recombination-dependent exponential amplification PCR) were used to create libraries o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genome Research
سال: 1998
ISSN: 1088-9051,1549-5469
DOI: 10.1101/gr.8.8.779